lamc1 recombinant protein (Proteintech)
Proteintech is a verified supplier 
93
Structured Review
Proteintech
lamc1 recombinant protein
Lamc1 Recombinant Protein, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lamc1+recombinant+protein/LAMC1+Fusion+Protein/pmc09066104-80-16-23
Average 93 stars, based on 1 article reviews
Lamc1 Recombinant Protein, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lamc1+recombinant+protein/LAMC1+Fusion+Protein/pmc09066104-80-16-23
Average 93 stars, based on 1 article reviews
lamc1 recombinant protein - by Bioz Stars,
2026-09
93/100 stars
Images
1) Product Images from "LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis"
Article Title: LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis
Journal: International Journal of Biological Sciences
doi: 10.7150/ijbs.70524
Figure Legend Snippet: Correlation between the density of LAMC1 at gastric cancer and clinicopathologic parameters
Techniques Used: Expressing
Figure Legend Snippet: Gastric cancer with high expression of LAMC1 have higher migration and invasion ability in primary site. (A) Expression of LAMC1 in gastric cancer cell lines was analyzed by ELISA and Western blots. (B) Western blots were used for E-cadherin and vimentin expression in gastric cancer cells with LAMC1 knockout. (C) Transwell assay in gastric cancer cells with LAMC1 knockout. (D) The morphological characteristics of tumor xenograft model in AGS NC/si group, and tumor weight and volume were shown. (E) The HE staining and immunohistochemical results of xenograft tumor in AGS NC/si group.
Techniques Used: Expressing, Migration, Enzyme-linked Immunosorbent Assay, Western Blot, Knock-Out, Transwell Assay, Staining, Immunohistochemical staining
Figure Legend Snippet: LAMC1-mediated preadipocytes differentiation promotes pre-metastatic niche formation and gastric cancer cell colonization in peritoneal microenvironment. (A) The Oil Red O staining and Western bolts for analyzing effect of different concentrations of LAMC1 (0, 50ng/ml and 100ng/ml) on 3T3-L1 and human omental preadipocytes differentiation for 5 days. (B) Cytokines secreted by 3T3-L1 after induction of different concentrations of LAMC1 for 24h were measured by RT-qPCR . ( C ) HSL expression in induced 3T3-L1 detected by RT-qPCR and western blots. (D) ELISA was used for analyzed FFAs, lipid droplets, adiponectin and leptin in 3T3-L1 CM after stimulation by different concentrations of LAMC1 for 4 days. (E) ELISA was used for analyzed FFAs in human omental preadipocytes CM after stimulation by different concentrations of LAMC1 for 4 days. (F) The flow chart of coculture. (G) ELISA for analyzing LAMC1 levels in supernatant of gastric cancer cell lines transfected with siLAMC1. (H) The lipid droplet formation ability of 3T3-L1 was measured by Oil Red O staining and adipocyte differentiation related protein after coculture for 5 days with gastric cancer cell supernatant transfected siLAMC1 for 48h. (I) Flowchart of reverse co-culture of 3T3-L1 supernatant and gastric cancer cells. (J) Western blots were used for E-cadherin and vimentin expression in gastric cells after coculture for 48h with 3T3-L1 supernatant induced by LAMC1 (0, 50ng/ml and 100ng/ml) for 4 days. (K) CCK8 assay for AGS cell proliferation detection . (L) The morphological characteristics of tumor xenograft model in AGS NC/si and 3T3-L1 coculture group, and tumor weight were shown. (M) The HE staining and immunohistochemical results of xenograft tumor in AGS NC/si and 3T3-L1 coculture group. Error bars, SD. *P < 0.05; **P < 0.01; ***P < 0.001.
Techniques Used: Staining, Western Blot, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Transfection, Co-Culture Assay, CCK-8 Assay, Immunohistochemical staining
Figure Legend Snippet: Differentiated preadipocytes remodel metabolic programming of gastric cancer cells. (A) Electron microscope was used to observe the content of lipid droplets in AGS cells after coculture for 48h with 3T3-L1 supernatant induced by LAMC1 (0, 50ng/ml and 100ng/ml) for 4 days. Blank group meant AGS cells weren't cocultured with 3T3-L1 supernatant. (B-D) The results of extracellular acidification, fatty acid oxidation and extracellular O 2 consumption in AGS cells after reverse coculture for 48h was shown. (E) The ratio of NADPH / NADP+ in AGS cells after reverse coculture for 48h was assessed as materials and method. (F) The ATP content in AGS cells after reverse coculture for 48h was shown. (G) AGS cells treated with 0.5mM palmitic acid for 48h were analyzed by mass spectrometry. The Heat map of differential genes was shown. (H) AGS cells treated with 0.5mM palmitic acid for 48h were analyzed by mass spectrometry. The Gene function classification of differential genes was shown. (I) The RT- qPCR was for analyzing expression of metabolism-related genes in AGS cells after treatment with 0.5mM palmitic acid or reverse coculture with 3T3-L1 CM for 24h. 10uM DC260126 was used to inhibit the effect of palmitic acid. (J) The immunohistochemical results of xenograft tumor in AGS NC/si and 3T3-L1 coculture group. Error bars, SD. *P < 0.05; **P < 0.01; ***P < 0.001.
Techniques Used: Microscopy, Mass Spectrometry, Quantitative RT-PCR, Expressing, Immunohistochemical staining
Figure Legend Snippet: Palmitic acid phosphorylates STAT3 and promotes LAMC1 secretion through miR-193a-3p. (A) AGS cells treated with 0.5mM palmitic acid for 48h was used for mass spectrometry analysis and the three most abundant pathway proteins were shown. (B) The western blots were for analyzing pathway protein expression in AGS cells after treatment with 0.5mM palmitic acid for 48h or reverse coculture with 3T3-L1 supernatant induced by 0 and 100ng/ml LAMC1 for 48h. 10uM DC260126 was used to inhibit the effect of palmitic acid. (C) AGS cells transfected with siSTAT3 had a low LAMC1 expression determined by RT-qPCR, Western blots. (D) LAMC1 expression in supernatant of AGS cells transfected with siSTAT3. (E) Flowchart of co-culture of gastric cancer cell supernatant and 3T3-L1. (F) The gastric cancer cell supernatant transfected with siSTAT3 for 48h was collected to coculture with 3T3-L1 for 5 days. Western blots and the Oil Red O staining were used for lipid formation ability. (G) Three independent miRNA target databases were used to predict the potential miRNAs. (H) RT-qPCR was for analyzing miR193a-3p, miR384 and miR448 expression levels in AGS cells with STAT3 overexpression. (I) The gastric cancer cell supernatant transfected with miR-193a-3p mimic, inhibitor or NC for 48h was collected to coculture with 3T3-L1 for 5 days, and the Oil Red O staining and Western blots were used for lipid formation ability. Error bars, SD. *P < 0.05; **P < 0.01; ***P < 0.001.
Techniques Used: Mass Spectrometry, Western Blot, Expressing, Transfection, Quantitative RT-PCR, Co-Culture Assay, Staining, Over Expression
Figure Legend Snippet: The miR-193a-3p inhibits LAMC1 expression in a post-transcriptional manner. (A-B) AGS cells were transfected with miR-193a-3p mimic, inhibitor or NC, and agarose gel electrophoresis and RT-qPCR were used to measure RNA level change, Western blots and ELISA for protein level. (C) The AGS cells were transfected with siLAMC1, RT-qPCR for miR-193a-3p expression. (D) A structure diagram of the pmirGLO dual-luciferase reporter vector with 3′-UTR of LAMC1 mRNA harbors miR-193a-3p binding sites. (E) The relative luciferase activity of reporter plasmids carrying wild-type or mutant LAMC1 3′-UTR. (F) The LAMC1 mRNA decay curves of AGS cells carrying miR-193a-3p mimic or mimic NC. (G) The AGS cells were transfected with miR-193a-3p mimic or mimic NC for 48h, and AGO2-RNA immunoprecipitation assay (RIP) was used for analyzed the combination method of miR-193a-3p and LAMC1. Error bars, SD. *P < 0.05; **P < 0.01; ***P < 0.001.
Techniques Used: Expressing, Transfection, Agarose Gel Electrophoresis, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Luciferase, Plasmid Preparation, Binding Assay, Activity Assay, Mutagenesis, RNA Immunoprecipitation
Figure Legend Snippet: Gastric cancer with high LAMC1 expression has high risk of peritoneal metastasis. (A) Gross anatomy and nodule numbers of abdominal cavity in nude mice and the red arrows indicated micrometastases in the gastric cancer peritoneal metastasis model. (B) ROC curve. (C) The mechanism diagram of LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis.
Techniques Used: Expressing
Related Articles
Recombinant:Article Title: LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis Article Snippet: For 3T3-L1 and human preadipocytes induction, the medium of cells was cultivated with DMEM containing 10% FBS, 10ug/ml insulin, 1umol/L dexamethasone, 0.5mmol/L 3-isobutyl-1-methylaxanthine (IBMX, HY-12318; MCE) for 2 days after 2 days of contact inhibition, and subsequently, the medium was replaced with high glucose DMEM medium containing 10ug/ml insulin and 10% FBS for 2 days, then, change the medium every day with DMEM supplemented with 10% FBS until 3T3-L1 and human preadipocytes were induced into mature adipocyte . .. In order to confirm the effect of LAMC1 on the differentiation of adipocytes, different concentrations of Saline:Article Title: LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis Article Snippet: For 3T3-L1 and human preadipocytes induction, the medium of cells was cultivated with DMEM containing 10% FBS, 10ug/ml insulin, 1umol/L dexamethasone, 0.5mmol/L 3-isobutyl-1-methylaxanthine (IBMX, HY-12318; MCE) for 2 days after 2 days of contact inhibition, and subsequently, the medium was replaced with high glucose DMEM medium containing 10ug/ml insulin and 10% FBS for 2 days, then, change the medium every day with DMEM supplemented with 10% FBS until 3T3-L1 and human preadipocytes were induced into mature adipocyte . .. In order to confirm the effect of LAMC1 on the differentiation of adipocytes, different concentrations of |